A review of the clinical records at our institute revealed four patients with clinical signs of dacryocystitis and a positive conjunctival culture for Sporothrix who were diagnosed with Sporothrix dacryocystitis. Context in source publication Context In the first serological survey , in which indirect immu- nofluorescence IIF tests were performed on blood collected on filter paper, 89 In the second survey conducted using the same technique in , 90 of the total samples Therefore, of the 2, tests performed, the average positivity rate was When we showed a collection of triatomines of the genera Triatoma , Panstrogylus and Rhodnius to a group of patients who we interviewed, Among the patients who recog- nised the triatomines, Among the individuals who reported previous attacks by triatomines, As a result of this previous study and due to our great- er experience with Chagas disease in the area Coura et al.
This staining led to the high prevalence of positivity Location of the study area - The administrative dis- trict of Barcelos is located north of AM. The city of Barcelos, where this study was conducted, is located on the right bank of the Negro River, km from Manaus, the capital of AM Fig. Survey and samples - The new serological survey was conducted in the main settlement of Barcelos and covered the entire resident population that was willing to participate in the survey after providing informed consent.
For minors, the informed consent was provided by their parents or guardians. The teams were trained by the project coordinator JR Coura, a physician and infec- tologist and were monitored throughout the survey by a postgraduate student MHP Marquez. Each team was composed of two health agents; the two laboratory tech- nicians each supervised three of the teams and all six teams were monitored by the postgraduate student. In this kit, the antigens used are cultured epimastigote forms of T.
The slides were prepared in accordance with the classi- cal techniques for IIF and were examined under an im- munofluorescence microscope by at least two observers. The slide readings were considered to be negative unre- active, absence of fluorescence , doubtful weakly reac- tive, low intensity of fluorescence or positive strongly reactive, high intensity of fluorescence.
After separation, the serum was frozen, packed on dry ice and sent from Barcelos to our laboratory in RJ. Samples were considered to be seronegative if two or three of the tests were negative and seropositive if two or three of the tests were positive.
